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專利授權區
專利名稱(中)	用於在活體外測定三維腫瘤球體中葡萄糖濃度的方法
專利名稱(英)	METHOD FOR DETERMINING GLUCOSE CONCENTRATION IN THREE-DIMENSIONAL TUMOR SPHEROID IN VITRO
專利家族	中華民國：I919395 美國：US2026/0056129A1(公開號)
專利權人	國立清華大學 100.00%
發明人	曾繁根,戴惠美
技術領域	生化醫藥

專利摘要(中)
一種用於在活體外測定三維腫瘤球體中之葡萄糖濃度的方法，包括：對癌細胞與基質細胞的共培養物添加表面-增強的拉曼散射(SERS)-為基礎的奈米感測器，俾以形成一第一混合物；將該第一混合物添加至聚二甲基矽氧烷(PDMS)-為基礎的微孔陣列晶片之微孔中；令該PDMS-為基礎的微孔陣列晶片中的第一混合物在37℃下進行培育，俾以形成嵌入有SERS-為基礎的葡萄糖奈米感測器的三維腫瘤球體；將該三維腫瘤球體轉移到載玻片上，而使得該三維腫瘤球體被封固於其上；令該封固在載玻片上的三維腫瘤球體進行共軛焦拉曼光譜學分析，俾以獲得該三維腫瘤球體的拉曼映射影像；以及自該拉曼映射影像中擷取拉曼強度並將該拉曼強度映射到一校準曲線，俾以測定在活體外三維腫瘤球體中的葡萄糖濃度。

專利摘要(中)

一種用於在活體外測定三維腫瘤球體中之葡萄糖濃度的方法，包括：對癌細胞與基質細胞的共培養物添加表面-增強的拉曼散射(SERS)-為基礎的奈米感測器，俾以形成一第一混合物；將該第一混合物添加至聚二甲基矽氧烷(PDMS)-為基礎的微孔陣列晶片之微孔中；令該PDMS-為基礎的微孔陣列晶片中的第一混合物在37℃下進行培育，俾以形成嵌入有SERS-為基礎的葡萄糖奈米感測器的三維腫瘤球體；將該三維腫瘤球體轉移到載玻片上，而使得該三維腫瘤球體被封固於其上；令該封固在載玻片上的三維腫瘤球體進行共軛焦拉曼光譜學分析，俾以獲得該三維腫瘤球體的拉曼映射影像；以及自該拉曼映射影像中擷取拉曼強度並將該拉曼強度映射到一校準曲線，俾以測定在活體外三維腫瘤球體中的葡萄糖濃度。

專利摘要(英)
A method for determining glucose concentration in a three-dimensional tumor spheroid in vitro includes: adding a surface-enhanced Raman spectroscopy (SERS)-based glucose nanosensor to a co-culture of cancer cells and stromal cells so as to form a first mixture; adding the first mixture into microwells of a polydimethylsiloxane(PDMS)-based microwell array chip; subjecting the first mixture in the PDMS-based microwell array chip to incubation at 37°C so as to form the three-dimensional tumor spheroid with the SERS-based glucose nanosensor embedded therein; transferring the three-dimensional tumor spheroid onto a glass slide to allow the three-dimensional tumor spheroid to be mounted thereon; subjecting the three-dimensional tumor spheroid to confocal Raman spectroscopy so as to obtain Raman mapping images of the three-dimensional tumor spheroid; and extracting Raman intensities from the Raman mapping images and mapping the Raman intensities to a calibration curve so as to determine glucose concentration in the three-dimensional tumor spheroid in vitro.

專利摘要(英)

A method for determining glucose concentration in a three-dimensional tumor spheroid in vitro includes: adding a surface-enhanced Raman spectroscopy (SERS)-based glucose nanosensor to a co-culture of cancer cells and stromal cells so as to form a first mixture; adding the first mixture into microwells of a polydimethylsiloxane(PDMS)-based microwell array chip; subjecting the first mixture in the PDMS-based microwell array chip to incubation at 37°C so as to form the three-dimensional tumor spheroid with the SERS-based glucose nanosensor embedded therein; transferring the three-dimensional tumor spheroid onto a glass slide to allow the three-dimensional tumor spheroid to be mounted thereon; subjecting the three-dimensional tumor spheroid to confocal Raman spectroscopy so as to obtain Raman mapping images of the three-dimensional tumor spheroid; and extracting Raman intensities from the Raman mapping images and mapping the Raman intensities to a calibration curve so as to determine glucose concentration in the three-dimensional tumor spheroid in vitro.

聯絡資訊
承辦人姓名	楊慧敏
承辦人電話	(03)571-5131 #31181
承辦人Email	hmyang@mx.nthu.edu.tw