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專利授權區
專利名稱(中)	大腸桿菌基因編輯系統及其基因編輯方法
專利名稱(英)	GENE EDITING SYSTEM OF ESCHERICHIA COLI AND GENE EDITING METHOD THEREOF
專利家族	中華民國：I821883 美國：US-2023-0235338-A1(公開號)
專利權人	國立清華大學 100%
發明人	胡育誠,張晉維,黃靖雯,周俊彥
技術領域	生化醫藥

專利摘要(英)
The invention relates to a gene editing system of Escherichia coli including a helper plasmid and a donor plasmid. The helper plasmid includes a transposase complex expression cassette, a Cas12k expression cassette, a first sgRNA cassette, a first antibiotic resistance gene and first replication origin. The donor plasmid includes a left end sequence of the ShCAST transposon, an exogenous gene expression cassette, a right end sequence of the ShCAST transposon, a second sgRNA cassette, a second antibiotic resistance gene and a second replication origin. The gene editing system of Escherichia coli can easily and effectively integrate large fragments of DNA sequences into target chromosome without causing double-strand breaks in the genome of Escherichia coli, so as to regulate the metabolic pathways of Escherichia coli strains useful in different biotechnologies.

專利摘要(英)

The invention relates to a gene editing system of Escherichia coli including a helper plasmid and a donor plasmid. The helper plasmid includes a transposase complex expression cassette, a Cas12k expression cassette, a first sgRNA cassette, a first antibiotic resistance gene and first replication origin. The donor plasmid includes a left end sequence of the ShCAST transposon, an exogenous gene expression cassette, a right end sequence of the ShCAST transposon, a second sgRNA cassette, a second antibiotic resistance gene and a second replication origin. The gene editing system of Escherichia coli can easily and effectively integrate large fragments of DNA sequences into target chromosome without causing double-strand breaks in the genome of Escherichia coli, so as to regulate the metabolic pathways of Escherichia coli strains useful in different biotechnologies.

聯絡資訊
承辦人姓名	李佳玲
承辦人電話	03-5715131 #62300
承辦人Email	cl.lee@mx.nthu.edu.tw