The present invention provides a new strategy for preparing an activatable
polypeptide arrangement to produce cyclized proteins, characterized in that the
strategy can be used to produce cyclized interleukins. The strategy cyclizes the
interleukins via intein-mediated protein splicing to prepare functional active
interleukin to replace native interleukins in bio-industrial applications. In the
strategy, the linker sequence used to connect the N- and C-terminus of the target
protein sequence is not related to the protein splicing reaction, thus providing
flexibility in the design of cyclized polypeptide. |