The present disclosure provides a sensing circuit, a set of pre-amplifiers, and an operating method thereof. The set of pre-amplifiers includes a first pre-amplifier and a second pre-amplifier. The first pre-amplifier is coupled to a first input terminal of the sense amplifier. The second pre-amplifier is coupled to a second input terminal of the sense amplifier. he first pre-amplifier and the second pre-amplifier respectively performs a discharging operation to discharge the first input terminal and the second input terminal of the sense amplifier after the first input terminal and the second input terminal of the sense amplifier are charged to a predetermined voltage. One of the first pre-amplifier and the second pre-amplifier amplifies a voltage difference between the first input terminal and the second input terminal of the sense amplifier by terminating the discharging operation of another of the first pre-amplifier and the second pre-amplifier. |