A positioning and measuring system includes: an image scale supporting an object and having positioning mark sets and encoding pattern sets arranged in a two-dimensional array, each of the positioning mark sets including positioning marks, each of the encoding pattern sets including encoding patterns respectively disposed in gaps between the positioning marks; an image capturing device capturing measurement points of the object and an image scale to obtain composite images; a processor processing the composite images and determining one or multiple position relationships between the measurement points according to the encoding patterns and the positioning marks; and a driving mechanism, which is electrically connected to the processor and mechanically connected to the image capturing device or the image scale, and drives one of the image capturing device and the image scale to move relatively to the other of the image capturing device and the image scale. |