A tissue identification method is provided, including a preparation step and a detection step. The preparation step includes preparing a biosensor which includes a transistor and a response electrode. The response electrode is spaced apart from a gate of the transistor. The detection step includes disposing a biological tissue sample on the response electrode, and applying a pulse voltage that has tunable pulse width and pulse height to the response electrode, causing in a voltage difference between the response electrode and the gate, and measuring a detection current which generates from the transistor in pulse width as to obtain the first sensing indicator. In addition, a biosensor for tissue identification is also provided. |