The present disclosure provides a perfusion cell culture device. The perfusion cell culture device includes a driving module and a plurality of cell culture modules. The driving module includes an opening for connecting a driving source and a chamber. The opening and the chamber are connected. Each of the cell culture modules includes a fluid channel, a first elastic element, two flow direction controlling units and a cell culture zone. The fluid channel is disposed above the chamber. The first elastic element is disposed between the fluid channel and the chamber. The two control units are disposed on two ends of the fluid channel respectively and connect the fluid channel selectively. The cell culture zone connects the two control units. Thus, the perfusion cell culture device of the present disclosure can perform multiple sets of perfusion cell culture by a single driving source that is favorable to achieve high throughput and simplify the system scale. |