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專利授權區
專利名稱(中)	維斯假絲酵母的基因編輯系統、其基因編輯方法、生產十二烷二酸的轉型株及生產十二烷二酸的方法
專利名稱(英)	GENE EDITING SYSTEM OF CANDIDA VISWANATHII, GENE EDITING METHOD THEREOF, TRANSFORMANT FOR PRODUCING DODECANEDIOIC ACID, AND METOHD FOR PRODUCING DODECANEDIOIC ACID
專利家族	中華民國：I857355
專利權人	國立清華大學 58.33% ,大連化學工業股份有限公司 10.42% ,長春人造樹脂廠股份有限公司 20.83% ,長春石油化學股份有限公司 10.42%
發明人	胡育誠,范玉南,周俊彥,王興運,劉珈男
技術領域	生化醫藥

專利摘要(英)
The invention relates to a gene editing system of Candida viswanathii including a Candida viswanathii, a first gene editing fragment, and a second gene editing fragment. The first gene editing fragment includes a first homology arm and a first selection gene fragment. The second gene editing fragment includes a second homology arm, a Cas9 expression cassette, and a sgRNA cassette. The Cas9 expression cassette includes a Cas9 promoter, a Cas9 gene, and three nuclear localization signals. The sgRNA cassette includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold sequence, and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment so as to perform gene editing on a chromosome of the Candida viswanathii. Therefore, every target chromosome of the Candida viswanathii can be edited easily so as to attain a stable recombinant without any specific condition.

專利摘要(英)

The invention relates to a gene editing system of Candida viswanathii including a Candida viswanathii, a first gene editing fragment, and a second gene editing fragment. The first gene editing fragment includes a first homology arm and a first selection gene fragment. The second gene editing fragment includes a second homology arm, a Cas9 expression cassette, and a sgRNA cassette. The Cas9 expression cassette includes a Cas9 promoter, a Cas9 gene, and three nuclear localization signals. The sgRNA cassette includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold sequence, and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment so as to perform gene editing on a chromosome of the Candida viswanathii. Therefore, every target chromosome of the Candida viswanathii can be edited easily so as to attain a stable recombinant without any specific condition.

聯絡資訊
承辦人姓名	李佳玲
承辦人電話	03-5715131 #62300
承辦人Email	cl.lee@mx.nthu.edu.tw