The present invention is related to a membrane electrochemical signal detection system, which comprises a detection platform and a probe, wherein the detection platform comprises a substrate having a cavity; a hydrogel layer disposed in the cavity of the substrate; and a carrier film disposed above the substrate and the hydrogel layer with at least one through hole corresponding to the cavity of the substrate as a sample slot. The surface of the probe is covered by an insulating layer and a metal for detection is exposed at a tip portion of the probe. |